› 6bjh

X-ray diffraction
2.58Å resolution

CIRV p19 mutant T111S in complex with siRNA

Released:
DOI: 10.2210/pdb6bjh/pdb
PDB entry 6bjh coloured by chain, front view.
PDB entry 6bjh coloured by chain, side view.
PDB entry 6bjh coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into interactions between viral suppressor of RNA silencing protein p19 mutants and small RNAs.
Foss DV, Schirle NT, MacRae IJ, Pezacki JP
FEBS Open Bio 9 1042-1051 (2019)
PMID: 31021526

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179245 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
RNA silencing suppressor p19 Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 19.34 KDa
Source organism: Carnation Italian ringspot virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q66104 (Residues: 1-172; Coverage: 100%)
Gene name: ORF4
Sequence domains: Tombusvirus P19 core protein
RNA (5'-R(P*UP*CP*GP*AP*AP*GP*UP*AP*UP*UP*CP*CP*GP*CP*GP*UP*AP*CP*GP*UP*U)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.67 KDa
Source organism: Lampyridae
Expression system: Not provided
RNA (5'-R(P*CP*GP*UP*AP*CP*GP*CP*GP*GP*AP*AP*UP*AP*CP*UP*UP*CP*GP*AP*UP*U)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.69 KDa
Source organism: Lampyridae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P1
Unit cell:
a: 45.788Å b: 46.219Å c: 54.181Å
α: 109.85° β: 112.11° γ: 95.52°
R-values:
R R work R free
0.227 0.224 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

An unnatural enzyme with endonuclease activity towards small non-coding RNAs.
Ahmed et al. (2023)
1 more

1 mention without citation

Structural insights into interactions between viral suppressor of RNA silencing protein p19 mutants and small RNAs.
Foss et al. (2019)