› 6bjx

X-ray diffraction
3.14Å resolution

Group I self-splicing intron P4-P6 domain mutant U131A (with isopropanol soaking)

Released:
DOI: 10.2210/pdb6bjx/pdb
PDB entry 6bjx coloured by chain, front view.
PDB entry 6bjx coloured by chain, side view.
PDB entry 6bjx coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
In Crystallo Selection to Establish New RNA Crystal Contacts.
Shoffner GM, Wang R, Podell E, Cech TR, Guo F
Structure 26 1275-1283.e3 (2018)
PMID: 29910185

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197030 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Group I self-splicing intron P4-P6 domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 158 nucleotides
Theoretical weight: 51.07 KDa
Source organism: Tetrahymena thermophila
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 20 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 74.67Å b: 130.01Å c: 146.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.229
Expression system: Not provided

Quick links

Citations

2 review citations

Computational modeling of RNA 3D structure based on experimental data.
Ponce-Salvatierra et al. (2019)
1 more

1 mention without citation

In Crystallo Selection to Establish New RNA Crystal Contacts.
Shoffner et al. (2018)