› 6bml

X-ray diffraction
2.95Å resolution

Structure of human DHHC20 palmitoyltransferase, irreversibly inhibited by 2-bromopalmitate

Released:
DOI: 10.2210/pdb6bml/pdb
PDB entry 6bml coloured by chain, front view.
PDB entry 6bml coloured by chain, side view.
PDB entry 6bml coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fatty acyl recognition and transfer by an integral membrane S-acyltransferase.
Rana MS, Kumar P, Lee CJ, Verardi R, Rajashankar KR, Banerjee A
Science 359 (2018)
PMID: 29326245

Function and Biology Details

Reaction catalysed: 
Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Palmitoyltransferase ZDHHC20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 34.6 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q5W0Z9 (Residues: 5-299; Coverage: 81%)
Gene name: ZDHHC20
Sequence domains: DHHC palmitoyltransferase

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand PLM 2 x PLM
Ligand PO4 2 x PO4
Ligand PAP 1 x PAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P63
Unit cell:
a: 99.794Å b: 99.794Å c: 159.947Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.236 0.245
Expression system: Komagataella pastoris

Quick links

Citations

27 review citations

Regulation of Dynamic Protein S-Acylation.
Chen et al. (2021)
26 more

10 mentions without citation

Inhibitors of DHHC family proteins.
Lan et al. (2021)
9 more