› 6bt2

X-ray diffraction
2.41Å resolution

Structure of the human Nocturnin catalytic domain with bound sulfate anion

Released:
DOI: 10.2210/pdb6bt2/pdb
PDB entry 6bt2 coloured by chain, front view.
PDB entry 6bt2 coloured by chain, side view.
PDB entry 6bt2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human Nocturnin reveals a conserved ribonuclease domain that represses target transcript translation and abundance in cells.
Abshire ET, Chasseur J, Bohn JA, Del Rizzo PA, Freddolino PL, Goldstrohm AC, Trievel RC
Nucleic Acids Res 46 6257-6270 (2018)
PMID: 29860338

Function and Biology Details

Reaction catalysed: 
NADP(+) + H(2)O = NAD(+) + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nocturnin Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 35.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UK39 (Residues: 120-431; Coverage: 72%)
Gene names: CCR4, CCRN4L, NOC, NOCT
Sequence domains: Endonuclease/Exonuclease/phosphatase family

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 62.004Å b: 69.437Å c: 153.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Functions of the major abasic endonuclease (APE1) in cell viability and genotoxin resistance.
McNeill et al. (2020)
1 more

2 mentions without citation

The structure of human Nocturnin reveals a conserved ribonuclease domain that represses target transcript translation and abundance in cells.
Abshire et al. (2018)
1 more