› 6bza

X-ray diffraction
2.6Å resolution

Crystal structure of halogenase PltM in complex with phloroglucinol and FAD

Released:
DOI: 10.2210/pdb6bza/pdb
PDB entry 6bza coloured by chain, front view.
PDB entry 6bza coloured by chain, side view.
PDB entry 6bza coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Primary publication:
Unusual substrate and halide versatility of phenolic halogenase PltM.
Mori S, Pang AH, Thamban Chandrika N, Garneau-Tsodikova S, Tsodikov OV
Nat Commun 10 1255 (2019)
PMID: 30890712

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Halogenase PltM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 522 amino acids
Theoretical weight: 58.35 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4KCZ3 (Residues: 1-502; Coverage: 100%)
Gene names: PFL_2784, pltM
Sequence domains: Tryptophan halogenase

Ligands and Environments

3 bound ligands:
Ligand 13X 3 x 13X
Ligand FAD 2 x FAD
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 64.242Å b: 156.98Å c: 213.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 citation in other articles

A marine viral halogenase that iodinates diverse substrates.
Gkotsi et al. (2019)
9 more

4 mentions without citation

Halogenases: a palette of emerging opportunities for synthetic biology-synthetic chemistry and C-H functionalisation.
Crowe et al. (2021)
3 more