› 6c1y

X-ray diffraction
2.3Å resolution

mbd of human mecp2 in complex with methylated DNA

Released:
DOI: 10.2210/pdb6c1y/pdb
PDB entry 6c1y coloured by chain, front view.
PDB entry 6c1y coloured by chain, side view.
PDB entry 6c1y coloured by chain, top view.
Source organisms:
Primary publication:
Plasticity at the DNA recognition site of the MeCP2 mCG-binding domain.
Lei M, Tempel W, Chen S, Liu K, Min J
Biochim Biophys Acta Gene Regul Mech 1862 194409 (2019)
PMID: 31356990

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119648 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Methyl-CpG-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 86 amino acids
Theoretical weight: 9.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51608 (Residues: 80-164; Coverage: 18%)
Gene name: MECP2
Sequence domains: Methyl-CpG binding domain
Structure domains: Methyl-cpg-binding Protein 2; Chain A
12-mer DNA Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand UNX 3 x UNX
1 modified residue:
Ligand 5CM 4 x 5CM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 40.753Å b: 47.359Å c: 54.623Å
α: 68.15° β: 89.83° γ: 65.98°
R-values:
R R work R free
0.233 0.231 0.286
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Emerging Insights into the Distinctive Neuronal Methylome.
Clemens et al. (2020)
10 other articles