› 6c50

X-ray diffraction
2.5Å resolution

Cross-alpha Amyloid-like Structure alphaAmS

Released:
DOI: 10.2210/pdb6c50/pdb
PDB entry 6c50 coloured by chain, front view.
PDB entry 6c50 coloured by chain, side view.
PDB entry 6c50 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Designed peptides that assemble into cross-α amyloid-like structures.
Zhang SQ, Huang H, Yang J, Kratochvil HT, Lolicato M, Liu Y, Shu X, Liu L, DeGrado WF
Nat Chem Biol 14 870-875 (2018)
PMID: 30061717

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo 236-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cross-alpha Amyloid-like Structure alphaAmS Chains: 11, 12, 13, 14, 21, 22, 23, 24, 31, 32, 33, 34, 41, 42, 43, 44, 51, 52, 53, 54, 61, 62, 63, 64, 71, 72, 73, 74, 81, 82, 83, 84, 91, 92, 93, 94, A1, A2, A3, A4, B1, B2, B3, B4, C1, C2, C3, C4, D1, D2, D3, D4, E1, E2, E3, E4, F1, F2, F3, F4, G1, G2, G3, G4, H1, H2, H3, H4, I1, I2, I3, I4, J1, J2, J3, J4, K1, K2, K3, K4, L1, L2, L3, L4, M1, M2, M3, M4, N1, N2, N3, N4, O1, O2, O3, O4, P1, P2, P3, P4, Q1, Q2, Q3, Q4, R1, R2, R3, R4, S1, S2, S3, S4, T1, T2, T3, T4, U1, U2, U3, U4, V1, V2, V3, V4, W1, W2, W3, W4, X1, X2, X3, X4, Y1, Y2, Y3, Y4, Z1, Z2, Z3, Z4, a1, a2, a3, a4, b1, b2, b3, b4, c1, c2, c3, c4, d1, d2, d3, d4, e1, e2, e3, e4, f1, f2, f3, f4, g1, g2, g3, g4, h1, h2, h3, h4, i1, i2, i3, i4, j1, j2, j3, j4, k1, k2, k3, k4, l1, l2, l3, l4, m1, m2, m3, m4, n1, n2, n3, n4, o1, o2, o3, o4, p1, p2, p3, p4, q1, q2, q3, q4, r1, r2, r3, r4, s1, s2, s3, s4, t1, t2, t3, t4, u1, u2, u3, u4, v1, v2, v3, v4, w1, w2, w3, w4, x1, x2, x3, x4
Molecule details ›
Chains: 11, 12, 13, 14, 21, 22, 23, 24, 31, 32, 33, 34, 41, 42, 43, 44, 51, 52, 53, 54, 61, 62, 63, 64, 71, 72, 73, 74, 81, 82, 83, 84, 91, 92, 93, 94, A1, A2, A3, A4, B1, B2, B3, B4, C1, C2, C3, C4, D1, D2, D3, D4, E1, E2, E3, E4, F1, F2, F3, F4, G1, G2, G3, G4, H1, H2, H3, H4, I1, I2, I3, I4, J1, J2, J3, J4, K1, K2, K3, K4, L1, L2, L3, L4, M1, M2, M3, M4, N1, N2, N3, N4, O1, O2, O3, O4, P1, P2, P3, P4, Q1, Q2, Q3, Q4, R1, R2, R3, R4, S1, S2, S3, S4, T1, T2, T3, T4, U1, U2, U3, U4, V1, V2, V3, V4, W1, W2, W3, W4, X1, X2, X3, X4, Y1, Y2, Y3, Y4, Z1, Z2, Z3, Z4, a1, a2, a3, a4, b1, b2, b3, b4, c1, c2, c3, c4, d1, d2, d3, d4, e1, e2, e3, e4, f1, f2, f3, f4, g1, g2, g3, g4, h1, h2, h3, h4, i1, i2, i3, i4, j1, j2, j3, j4, k1, k2, k3, k4, l1, l2, l3, l4, m1, m2, m3, m4, n1, n2, n3, n4, o1, o2, o3, o4, p1, p2, p3, p4, q1, q2, q3, q4, r1, r2, r3, r4, s1, s2, s3, s4, t1, t2, t3, t4, u1, u2, u3, u4, v1, v2, v3, v4, w1, w2, w3, w4, x1, x2, x3, x4
Length: 27 amino acids
Theoretical weight: 2.95 KDa
Source organism: synthetic construct
Expression system: synthetic construct

Ligands and Environments

2 bound ligands:
Ligand FMT 97 x FMT
Ligand MPD 6 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 161.166Å b: 160.159Å c: 198.502Å
α: 90° β: 109.97° γ: 90°
R-values:
R R work R free
0.2 0.198 0.246
Expression system: synthetic construct

Quick links

Citations

4 review citations

Recent advances in de novo protein design: Principles, methods, and applications.
Pan et al. (2021)
3 more

1 mention without citation

Designed peptides that assemble into cross-α amyloid-like structures.
Zhang et al. (2018)