› 6c6s

Electron Microscopy
3.7Å resolution

CryoEM structure of E.coli RNA polymerase elongation complex bound with RfaH

Released:
DOI: 10.2210/pdb6c6s/pdb
PDB entry 6c6s coloured by chain, front view.
PDB entry 6c6s coloured by chain, side view.
PDB entry 6c6s coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Basis for Transcript Elongation Control by NusG Family Universal Regulators.
Kang JY, Mooney RA, Nedialkov Y, Saba J, Mishanina TV, Artsimovitch I, Landick R, Darst SA
Cell 173 1650-1662.e14 (2018)
PMID: 29887376
Related structures: EMD-7349

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-119653 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: G, H
Molecule details ›
Chains: G, H
Length: 239 amino acids
Theoretical weight: 26.46 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-234; Coverage: 71%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta Chain: I
Molecule details ›
Chain: I
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: J
Molecule details ›
Chain: J
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: K
Molecule details ›
Chain: K
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
Transcription antitermination protein RfaH Chain: D
Molecule details ›
Chain: D
Length: 162 amino acids
Theoretical weight: 18.36 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFW0 (Residues: 1-162; Coverage: 100%)
Gene names: JW3818, b3842, hlyT, rfaH, sfrB
Sequence domains: Transcription termination factor nusG
DNA (29-MER) Chain: A
Molecule details ›
Chain: A
Length: 29 nucleotides
Theoretical weight: 9 KDa
DNA (29-MER) Chain: B
Molecule details ›
Chain: B
Length: 29 nucleotides
Theoretical weight: 8.84 KDa
RNA (5'-R(*GP*CP*AP*UP*UP*CP*AP*AP*AP*GP*CP*CP*GP*AP*GP*AP*GP*GP*UP*A)-3') Chain: R
Molecule details ›
Chain: R
Length: 20 nucleotides
Theoretical weight: 6.47 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-7349
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Organization and regulation of gene transcription.
Cramer P. (2019)
17 more

12 mentions without citation

Functional and Regulatory Roles of Fold-Switching Proteins.
Kim et al. (2021)
11 more