› 6cd7

X-ray diffraction
1.53Å resolution

Crystal structure of APH(2")-IVa in complex with plazomicin

Released:
DOI: 10.2210/pdb6cd7/pdb
PDB entry 6cd7 coloured by chain, front view.
PDB entry 6cd7 coloured by chain, side view.
PDB entry 6cd7 coloured by chain, top view.
Source organism: Enterococcus casseliflavus
Primary publication:
Plazomicin Retains Antibiotic Activity against Most Aminoglycoside Modifying Enzymes.
Cox G, Ejim L, Stogios PJ, Koteva K, Bordeleau E, Evdokimova E, Sieron AO, Savchenko A, Serio AW, Krause KM, Wright GD
ACS Infect Dis 4 980-987 (2018)
PMID: 29634241

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 35.48 KDa
Source organism: Enterococcus casseliflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: O68183 (Residues: 1-301; Coverage: 100%)
Gene name: aph(2'')-Id
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand EDS 1 x EDS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 43.455Å b: 101.813Å c: 72.921Å
α: 90° β: 98.47° γ: 90°
R-values:
R R work R free
0.173 0.171 0.208
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Antimicrobial Resistance in ESKAPE Pathogens.
De Oliveira et al. (2020)
20 more

1 mention without citation

Plazomicin Retains Antibiotic Activity against Most Aminoglycoside Modifying Enzymes.
Cox et al. (2018)