› 6clb

Electron crystallography
3.2Å resolution

3.20 A MicroED structure of proteinase K at 7.8 e- / A^2

Released:
DOI: 10.2210/pdb6clb/pdb
PDB entry 6clb coloured by chain, front view.
PDB entry 6clb coloured by chain, side view.
PDB entry 6clb coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.
Hattne J, Shi D, Glynn C, Zee CT, Gallagher-Jones M, Martynowycz MW, Rodriguez JA, Gonen T
Structure 26 759-766.e4 (2018)
PMID: 29706530
Related structures: EMD-7494

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-7494

Quick links

Citations

17 review citations

Challenges and opportunities in cryo-EM single-particle analysis.
Lyumkis D. (2019)
16 more

1 mention without citation

Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.
Hattne et al. (2018)