› 6cob

X-ray diffraction
1.82Å resolution

AtHNL enantioselectivity mutant At-A9-H7 Apo, Y13C,Y121L,P126F,L128W,C131T,F179L,A209I

Released:
DOI: 10.2210/pdb6cob/pdb
PDB entry 6cob coloured by chain, front view.
PDB entry 6cob coloured by chain, side view.
PDB entry 6cob coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Jones BJ, Kazlauskas RJ, Desrouleaux R

Function and Biology Details

Reaction catalysed: 
(R)-mandelonitrile = cyanide + benzaldehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-hydroxynitrile lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 29.44 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LFT6 (Residues: 1-258; Coverage: 100%)
Gene names: At5g10300, F18D22_70, HNL, MES5
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 49.982Å b: 87.11Å c: 123.356Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.187
Expression system: Escherichia coli BL21(DE3)

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