› 6cpv

Electron crystallography
2.5Å resolution

MicroED structure of NaK ion channel reveals a process of Na+ partition into the selectivity filter

Released:
DOI: 10.2210/pdb6cpv/pdb
PDB entry 6cpv coloured by chain, front view.
PDB entry 6cpv coloured by chain, side view.
PDB entry 6cpv coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
MicroED structure of the NaK ion channel reveals a Na+ partition process into the selectivity filter.
Liu S, Gonen T
Commun Biol 1 38 (2018)
PMID: 30167468
Related structures: EMD-7558

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.71 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81HW2 (Residues: 19-110; Coverage: 81%)
Gene name: BC_0669
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand NA 12 x NA
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.5Å
Relevant EMDB volumes: EMD-7558
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The cryo-EM method microcrystal electron diffraction (MicroED).
Nannenga et al. (2019)
8 more

6 mentions without citation

Beyond protein structure determination with MicroED.
Nguyen et al. (2020)
5 more