› 6csx

Electron Microscopy
3Å resolution

Single particles Cryo-EM structure of AcrB D407A associated with lipid bilayer at 3.0 Angstrom

Released:
DOI: 10.2210/pdb6csx/pdb
PDB entry 6csx coloured by chain, front view.
PDB entry 6csx coloured by chain, side view.
PDB entry 6csx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure and activity of lipid bilayer within a membrane-protein transporter.
Qiu W, Fu Z, Xu GG, Grassucci RA, Zhang Y, Frank J, Hendrickson WA, Guo Y
Proc Natl Acad Sci U S A 115 12985-12990 (2018)
PMID: 30509977
Related structures: EMD-7609

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1057 amino acids
Theoretical weight: 114.69 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

2 bound ligands:
Ligand PTY 18 x PTY
Ligand D12 1 x D12
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-7609
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
25 more

5 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
4 more