› 6ct7

X-ray diffraction
1.9Å resolution

Fab of anti-a-synuclein antibody BIIB054 in complex with acetylated a-synuclein peptide (1-10)

Released:
DOI: 10.2210/pdb6ct7/pdb
PDB entry 6ct7 coloured by chain, front view.
PDB entry 6ct7 coloured by chain, side view.
PDB entry 6ct7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Development of an aggregate-selective, human-derived α-synuclein antibody BIIB054 that ameliorates disease phenotypes in Parkinson's disease models.
Weihofen A, Liu Y, Arndt JW, Huy C, Quan C, Smith BA, Baeriswyl JL, Cavegn N, Senn L, Su L, Marsh G, Auluck PK, Montrasio F, Nitsch RM, Hirst WD, Cedarbaum JM, Pepinsky RB, Grimm J, Weinreb PH
Neurobiol Dis 124 276-288 (2019)
PMID: 30381260

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211188 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
BIIB054 Fab light chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 214 amino acids
Theoretical weight: 22.89 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
BIIB054 Fab heavy chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 220 amino acids
Theoretical weight: 23.32 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Alpha-synuclein Chains: S, T
Molecule details ›
Chains: S, T
Length: 11 amino acids
Theoretical weight: 1.18 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P37840 (Residues: 1-10; Coverage: 7%)
Gene names: NACP, PARK1, SNCA

Ligands and Environments

1 bound ligand:
Ligand IPA 4 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 72.849Å b: 101.492Å c: 73.4Å
α: 90° β: 117.3° γ: 90°
R-values:
R R work R free
0.182 0.18 0.223
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

44 review citations

Amyloid Oligomers: A Joint Experimental/Computational Perspective on Alzheimer's Disease, Parkinson's Disease, Type II Diabetes, and Amyotrophic Lateral Sclerosis.
Nguyen et al. (2021)
43 more