› 6cue

Electron Microscopy
4Å resolution

Cryo-EM structure at 4.0 A resolution of vaccine-elicited antibody vFP7.04 in complex with HIV-1 Env BG505 DS-SOSIP, and antibodies VRC03 and PGT122

Released:
DOI: 10.2210/pdb6cue/pdb
PDB entry 6cue coloured by chain, front view.
PDB entry 6cue coloured by chain, side view.
PDB entry 6cue coloured by chain, top view.
Source organisms:
Primary publication:
Complete functional mapping of infection- and vaccine-elicited antibodies against the fusion peptide of HIV.
Dingens AS, Acharya P, Haddox HK, Rawi R, Xu K, Chuang GY, Wei H, Zhang B, Mascola JR, Carragher B, Potter CS, Overbaugh J, Kwong PD, Bloom JD
PLoS Pathog 14 e1007159 (2018)
PMID: 29975771
Related structures: EMD-7621

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
PDBe Complex ID:
PDB-CPX-212193 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (13 distinct):
Envelope glycoprotein gp160 Chains: 2, C, c
Molecule details ›
Chains: 2, C, c
Length: 473 amino acids
Theoretical weight: 52.99 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-502; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: 1, D, d
Molecule details ›
Chains: 1, D, d
Length: 153 amino acids
Theoretical weight: 17.16 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S7 (Residues: 509-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
vFP7.04 Heavy chain Chains: 3, H, h
Molecule details ›
Chains: 3, H, h
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
vFP7.04 light chain Chains: 4, L, l
Molecule details ›
Chains: 4, L, l
Length: 112 amino acids
Theoretical weight: 12.26 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
PGT122 heavy Chain Chains: 5, M, m
Molecule details ›
Chains: 5, M, m
Length: 132 amino acids
Theoretical weight: 14.84 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PGT122 Light chain Chains: 6, N, n
Molecule details ›
Chains: 6, N, n
Length: 107 amino acids
Theoretical weight: 11.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
VRC03 Heavy chain Chains: 7, Q, q
Molecule details ›
Chains: 7, Q, q
Length: 129 amino acids
Theoretical weight: 14.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
VRC03 light chain Chains: 8, R, r
Molecule details ›
Chains: 8, R, r
Length: 102 amino acids
Theoretical weight: 11.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-7621
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Recent progress in broadly neutralizing antibodies to HIV.
Sok et al. (2018)
6 more

3 mentions without citation

Complete functional mapping of infection- and vaccine-elicited antibodies against the fusion peptide of HIV.
Dingens et al. (2018)
2 more