› 6d12

X-ray diffraction
2.2Å resolution

Crystal structure of C-terminal xRRM domain of human Larp7 bound to 7SK stem-loop 4 RNA

Released:
DOI: 10.2210/pdb6d12/pdb
PDB entry 6d12 coloured by chain, front view.
PDB entry 6d12 coloured by chain, side view.
PDB entry 6d12 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for recognition of human 7SK long noncoding RNA by the La-related protein Larp7.
Eichhorn CD, Yang Y, Repeta L, Feigon J
Proc Natl Acad Sci U S A 115 E6457-E6466 (2018)
PMID: 29946027

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175529 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
La-related protein 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 13.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4G0J3 (Residues: 445-556; Coverage: 19%)
Gene names: HDCMA18P, LARP7
Sequence domains: RNA binding motif
human 7SK RNA stem-loop 4 Chain: C
Molecule details ›
Chain: C
Length: 38 nucleotides
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 47.91Å b: 34.03Å c: 95.01Å
α: 90° β: 95.18° γ: 90°
R-values:
R R work R free
0.23 0.228 0.267
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Mechanisms of Long Non-Coding RNAs in Cancers and Their Dynamic Regulations.
Zhang et al. (2020)
3 more

14 mentions without citation

The La-related proteins: structures and interactions of a versatile superfamily of RNA-binding proteins.
Dock-Bregeon et al. (2021)
13 more