› 6d24

X-ray diffraction
3.35Å resolution

Trypanosoma cruzi Glucose-6-P Dehydrogenase in complex with G6P

Released:
DOI: 10.2210/pdb6d24/pdb
PDB entry 6d24 coloured by chain, front view.
PDB entry 6d24 coloured by chain, side view.
PDB entry 6d24 coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Glucose-6-Phosphate Dehydrogenase from the Human Pathogen Trypanosoma cruzi Evolved Unique Structural Features to Support Efficient Product Formation.
Ortíz C, Botti H, Buschiazzo A, Comini MA
J Mol Biol 431 2143-2162 (2019)
PMID: 30930048

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173071 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucose-6-phosphate 1-dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 541 amino acids
Theoretical weight: 60.95 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1WBU6 (Residues: 38-555; Coverage: 93%)
Gene names: C3747_142g63, G6PDH1
Sequence domains:
Structure domains:
Glucose-6-phosphate 1-dehydrogenase Chains: C, D
Molecule details ›
Chains: C, D
Length: 541 amino acids
Theoretical weight: 60.97 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1WBU6 (Residues: 38-555; Coverage: 93%)
Gene names: C3747_142g63, G6PDH1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand BG6 4 x BG6
Ligand SO4 17 x SO4
Ligand GOL 14 x GOL
Ligand CL 23 x CL
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 96.79Å b: 133.03Å c: 107.75Å
α: 90° β: 100.27° γ: 90°
R-values:
R R work R free
0.206 0.205 0.254
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Modeling and Structure Determination of Homo-Oligomeric Proteins: An Overview of Challenges and Current Approaches.
Gaber et al. (2021)
3 other articles

1 mention without citation

Crystal structure of Leishmania donovani glucose 6-phosphate dehydrogenase reveals a unique N-terminal domain.
Berneburg et al. (2022)