› 6d38

X-ray diffraction
2.8Å resolution

Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)

Released:
DOI: 10.2210/pdb6d38/pdb
PDB entry 6d38 coloured by chain, front view.
PDB entry 6d38 coloured by chain, side view.
PDB entry 6d38 coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Optical control of cell signaling by single-chain photoswitchable kinases.
Zhou XX, Fan LZ, Li P, Shen K, Lin MZ
Science 355 836-842 (2017)
PMID: 28232577

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.77 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand GYC 1 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P43212
Unit cell:
a: 52.039Å b: 52.039Å c: 163.621Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.195 0.263
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Exercise and Mitochondrial Dynamics: Keeping in Shape with ROS and AMPK.
Trewin et al. (2018)
36 more