› 6d71

X-ray diffraction
1.72Å resolution

Crystal Structure of the Human Miro1 N-terminal GTPase bound to GTP

Released:
DOI: 10.2210/pdb6d71/pdb
PDB entry 6d71 coloured by chain, front view.
PDB entry 6d71 coloured by chain, side view.
PDB entry 6d71 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insight into human Miro1/2 domain organization based on the structure of its N-terminal GTPase.
Smith KP, Focia PJ, Chakravarthy S, Landahl EC, Klosowiak JL, Rice SE, Freymann DM
J Struct Biol 212 107656 (2020)
PMID: 33132189

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184914 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial Rho GTPase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 21.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IXI2 (Residues: 2-180; Coverage: 29%)
Gene names: ARHT1, RHOT1
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 41.35Å b: 78.27Å c: 99.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.22
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Transmembrane Membrane Readers form a Novel Class of Proteins That Include Peripheral Phosphoinositide Recognition Domains and Viral Spikes.
Overduin et al. (2022)
2 more

5 mentions without citation

Miro: A molecular switch at the center of mitochondrial regulation.
Eberhardt et al. (2020)
4 more