› 6dc0

X-ray diffraction
2.8Å resolution

Tribbles (TRIB1) pseudokinase fused to CCAAT-enhancer binding protein (C/EBPalpha) degron

Released:
DOI: 10.2210/pdb6dc0/pdb
PDB entry 6dc0 coloured by chain, front view.
PDB entry 6dc0 coloured by chain, side view.
PDB entry 6dc0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Substrate binding allosterically relieves autoinhibition of the pseudokinase TRIB1.
Jamieson SA, Ruan Z, Burgess AE, Curry JR, McMillan HD, Brewster JL, Dunbier AK, Axtman AD, Kannan N, Mace PD
Sci Signal 11 (2018)
PMID: 30254053

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CCAAT/enhancer-binding protein alpha; Tribbles homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 32.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96RU8 (Residues: 90-343; Coverage: 68%)
  • Canonical: P49715 (Residues: 51-75; Coverage: 7%)
Gene names: C8FW, CEBP, CEBPA, GIG2, TRB1, TRIB1
Sequence domains: Protein kinase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P6122
Unit cell:
a: 98.809Å b: 98.809Å c: 332.705Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.22 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Control of Cell Growth and Proliferation by the Tribbles Pseudokinase: Lessons from Drosophila.
Dobens et al. (2021)
4 more

7 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
6 more