› 6dcr

X-ray diffraction
1.98Å resolution

E. coli PriA helicase winged helix domain deletion protein

Released:
DOI: 10.2210/pdb6dcr/pdb
PDB entry 6dcr coloured by chain, front view.
PDB entry 6dcr coloured by chain, side view.
PDB entry 6dcr coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure-specific DNA replication-fork recognition directs helicase and replication restart activities of the PriA helicase.
Windgassen TA, Leroux M, Satyshur KA, Sandler SJ, Keck JL
Proc Natl Acad Sci U S A 115 E9075-E9084 (2018)
PMID: 30201718

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Primosomal protein N' Chains: A, B
Molecule details ›
Chains: A, B
Length: 694 amino acids
Theoretical weight: 77.28 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P17888 (Residues: 1-114, 175-732; Coverage: 92%)
Gene names: JW3906, b3935, priA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 69.132Å b: 56.505Å c: 195.863Å
α: 90° β: 97.67° γ: 90°
R-values:
R R work R free
0.187 0.186 0.215
Expression system: Escherichia coli

Quick links

Citations

5 review citations

DNA Helicase-SSB Interactions Critical to the Regression and Restart of Stalled DNA Replication forks in Escherichia coli.
Bianco PR. (2020)
4 more

2 mentions without citation

Structure-specific DNA replication-fork recognition directs helicase and replication restart activities of the PriA helicase.
Windgassen et al. (2018)
1 more