6dfs

X-ray diffraction
3.1Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-209000 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
mouse TCR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 210 amino acids
Theoretical weight: 23.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Structure domains: Immunoglobulins
mouse TCR beta chain Chain: B
Molecule details ›
Chain: B
Length: 242 amino acids
Theoretical weight: 27.75 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Structure domains: Immunoglobulins
H-2 class II histocompatibility antigen, A-D alpha chain Chain: C
Molecule details ›
Chain: C
Length: 183 amino acids
Theoretical weight: 20.9 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04228 (Residues: 26-208; Coverage: 79%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
Ig-like domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 215 amino acids
Theoretical weight: 24.78 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q31135 (Residues: 21-217; Coverage: 77%)
Gene name: H2-Ab1
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P63
Unit cell:
a: 269.518Å b: 269.518Å c: 45.639Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.24 0.285
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Trichoplusia ni