› 6dks

X-ray diffraction
2.78Å resolution

Structure of the Rbpj-SHARP-DNA Repressor Complex

Released:
DOI: 10.2210/pdb6dks/pdb
PDB entry 6dks coloured by chain, front view.
PDB entry 6dks coloured by chain, side view.
PDB entry 6dks coloured by chain, top view.
Source organisms:
Primary publication:
Structural and Functional Studies of the RBPJ-SHARP Complex Reveal a Conserved Corepressor Binding Site.
Yuan Z, VanderWielen BD, Giaimo BD, Pan L, Collins CE, Turkiewicz A, Hein K, Oswald F, Borggrefe T, Kovall RA
Cell Rep 26 845-854.e6 (2019)
PMID: 30673607

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199490 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Recombining binding protein suppressor of hairless Chains: C, G
Molecule details ›
Chains: C, G
Length: 422 amino acids
Theoretical weight: 47.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P31266 (Residues: 53-474; Coverage: 80%)
Gene names: Igkjrb1, Igkrsbp, Rbpj, Rbpsuh
Sequence domains:
Structure domains:
Maltose/maltodextrin-binding periplasmic protein Chains: D, H
Molecule details ›
Chains: D, H
Length: 407 amino acids
Theoretical weight: 44.35 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 28-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3') Chains: A, E
Molecule details ›
Chains: A, E
Length: 15 nucleotides
Theoretical weight: 4.5 KDa
DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3') Chains: B, F
Molecule details ›
Chains: B, F
Length: 15 nucleotides
Theoretical weight: 4.67 KDa

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 54.45Å b: 231.57Å c: 90.26Å
α: 90° β: 99.88° γ: 90°
R-values:
R R work R free
0.197 0.195 0.228
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Chromatin Regulator SPEN/SHARP in X Inactivation and Disease.
Giaimo et al. (2021)
3 more

5 mentions without citation

Biophysics of Notch Signaling.
Sprinzak et al. (2021)
4 more