6du5

X-ray diffraction
3.01Å resolution

Crystal structure of hMettl16 catalytic domain in complex with MAT2A 3'UTR hairpin 6

Released:

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + adenine in U6 snRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in U6 snRNA
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-183227 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA N6-adenosine-methyltransferase METTL16 Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 36.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86W50 (Residues: 2-310; Coverage: 55%)
Gene names: METT10D, METTL16
Sequence domains: RNA methyltransferase
hp6-RNA (25-MER) Chain: B
Molecule details ›
Chain: B
Length: 27 nucleotides
Theoretical weight: 8.78 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 134.918Å b: 134.918Å c: 76.208Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.265
Expression systems:
  • Escherichia coli
  • Not provided