Function and Biology Details
Reactions catalysed:
Myristoyl-CoA + acetyl-CoA = 3-oxopalmitoyl-CoA + CoA
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
A long-chain (S)-3-hydroxyacyl-CoA + NAD(+) = a long-chain 3-oxoacyl-CoA + NADH
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Thiolase-like
- 3-hydroxyacyl-CoA dehydrogenase, C-terminal
- 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily
- Thiolase
- 3-hydroxyacyl-CoA dehydrogenase, conserved site
- 3-hydroxyacyl-CoA dehydrogenase, NAD binding
- NAD(P)-binding domain superfamily
- Enoyl-CoA hydratase/isomerase, conserved site
9 more domains
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
Mitochondrial trifunctional enzyme complex (preferred)
PDBe Complex ID:
PDB-CPX-154212 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trifunctional enzyme subunit beta, mitochondrial
Molecule details ›
Chains: A, B, C, D, E, F
Length: 457 amino acids
Theoretical weight: 49.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 457 amino acids
Theoretical weight: 49.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P55084 (Residues: 34-474; Coverage: 93%)
Sequence domains:
Trifunctional enzyme subunit alpha, mitochondrial
Molecule details ›
Chains: G, H, I, J, K, L
Length: 727 amino acids
Theoretical weight: 79.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 727 amino acids
Theoretical weight: 79.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P40939 (Residues: 37-763; Coverage: 95%)
Sequence domains:
- Enoyl-CoA hydratase/isomerase
- 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain
- 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 17-BM
Spacegroup:
P21
Expression system: Escherichia coli BL21(DE3)
