› 6dwy

X-ray diffraction
3.2Å resolution

Hermes transposase deletion dimer complex with (C/G) DNA and Ca2+

Released:
DOI: 10.2210/pdb6dwy/pdb
PDB entry 6dwy coloured by chain, front view.
PDB entry 6dwy coloured by chain, side view.
PDB entry 6dwy coloured by chain, top view.
Source organism: Musca domestica
Primary publication:
Structural insights into the mechanism of double strand break formation by Hermes, a hAT family eukaryotic DNA transposase.
Hickman AB, Voth AR, Ewis H, Li X, Craig NL, Dyda F
Nucleic Acids Res 46 10286-10301 (2018)
PMID: 30239795

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119819 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
BED-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 517 amino acids
Theoretical weight: 59.03 KDa
Source organism: Musca domestica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q25438 (Residues: 80-612; Coverage: 84%)
Sequence domains:
DNA (5'-D(*AP*GP*AP*GP*AP*AP*CP*AP*AP*CP*AP*AP*CP*AP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
Source organism: Musca domestica
Expression system: Not provided
DNA (26-MER) Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.98 KDa
Source organism: Musca domestica
Expression system: Not provided
DNA (5'-D(*C*GP*CP*GP*TP*GP*AP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Musca domestica
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 121.38Å b: 135.35Å c: 103.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.259 0.295
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Jump ahead with a twist: DNA acrobatics drive transposition forward.
Arinkin et al. (2019)
1 more

1 mention without citation

Structural insights into the mechanism of double strand break formation by Hermes, a hAT family eukaryotic DNA transposase.
Hickman et al. (2018)