› 6dx3

X-ray diffraction
2.05Å resolution

Crystal structure of the viral OTU domain protease from Taggert virus

Released:
DOI: 10.2210/pdb6dx3/pdb
PDB entry 6dx3 coloured by chain, front view.
PDB entry 6dx3 coloured by chain, side view.
PDB entry 6dx3 coloured by chain, top view.
Source organism: Taggert virus
Primary publication:
Probing the impact of nairovirus genomic diversity on viral ovarian tumor domain protease (vOTU) structure and deubiquitinase activity.
Dzimianski JV, Beldon BS, Daczkowski CM, Goodwin OY, Scholte FEM, Bergeron É, Pegan SD
PLoS Pathog 15 e1007515 (2019)
PMID: 30629698

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase L Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 175 amino acids
Theoretical weight: 20.53 KDa
Source organism: Taggert virus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A142J8F6 (Residues: 1-166; Coverage: 4%)
Sequence domains: OTU-like cysteine protease

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 47.163Å b: 60.948Å c: 66.501Å
α: 64.95° β: 89.84° γ: 84.31°
R-values:
R R work R free
0.228 0.227 0.25
Expression system: Escherichia coli

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Citations

4 review citations

HERC5 and the ISGylation Pathway: Critical Modulators of the Antiviral Immune Response.
Mathieu et al. (2021)
3 more

3 mentions without citation

Identification and characterization of diverse OTU deubiquitinases in bacteria.
Schubert et al. (2020)
2 more