› 6e12

X-ray diffraction
2.4Å resolution

Crystal Structure of the Alr8543 protein in complex with Oleic Acid and magnesium ion from Nostoc sp. PCC 7120, Northeast Structural Genomics Consortium Target NsR141

Released:
DOI: 10.2210/pdb6e12/pdb
PDB entry 6e12 coloured by chain, front view.
PDB entry 6e12 coloured by chain, side view.
PDB entry 6e12 coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Forouhar F, Neely H, Seetharaman J, Xiao R, Ciccosanti C, Patel D, Belote RL, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-187069 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquinone biosynthesis protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 26.05 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YJY7 (Residues: 1-217; Coverage: 100%)
Gene name: alr8543
Sequence domains: Coenzyme Q (ubiquinone) biosynthesis protein Coq4

Ligands and Environments

3 bound ligands:
Ligand OLA 2 x OLA
Ligand MG 2 x MG
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: I222
Unit cell:
a: 113.308Å b: 112.918Å c: 167.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.199
Expression system: Escherichia coli

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