› 6e3k

X-ray diffraction
3.25Å resolution

Interferon gamma signalling complex with IFNGR1 and IFNGR2

Released:
DOI: 10.2210/pdb6e3k/pdb
PDB entry 6e3k coloured by chain, front view.
PDB entry 6e3k coloured by chain, side view.
PDB entry 6e3k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the IFNγ receptor complex guides design of biased agonists.
Mendoza JL, Escalante NK, Jude KM, Sotolongo Bellon J, Su L, Horton TM, Tsutsumi N, Berardinelli SJ, Haltiwanger RS, Piehler J, Engleman EG, Garcia KC
Nature 567 56-60 (2019)
PMID: 30814731

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134402 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Interferon gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 17.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P01579 (Residues: 24-156; Coverage: 93%)
Gene name: IFNG
Sequence domains: Interferon gamma
Structure domains: Growth Hormone; Chain: A;
Interferon gamma receptor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 242 amino acids
Theoretical weight: 27.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P15260 (Residues: 18-246; Coverage: 49%)
Gene name: IFNGR1
Sequence domains:
Structure domains: Immunoglobulins
Interferon gamma receptor 2 Chains: E, I
Molecule details ›
Chains: E, I
Length: 233 amino acids
Theoretical weight: 26.31 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P38484 (Residues: 28-247; Coverage: 70%)
Gene names: IFNGR2, IFNGT1
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 12 x NAG
Ligand EDO 6 x EDO
Ligand CYS 2 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 78.284Å b: 151.464Å c: 211.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.232
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

15 review citations

Roles of IFN-γ in tumor progression and regression: a review.
Jorgovanovic et al. (2020)
14 more

7 mentions without citation

The Role of Structure in the Biology of Interferon Signaling.
Walter MR. (2020)
6 more