› 6e7b

Electron Microscopy
3.5Å resolution

13-pf 3-start GMPCPP-human alpha1B/beta3 microtubules

Released:
DOI: 10.2210/pdb6e7b/pdb
PDB entry 6e7b coloured by chain, front view.
PDB entry 6e7b coloured by chain, side view.
PDB entry 6e7b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human β-Tubulin Isotypes Can Regulate Microtubule Protofilament Number and Stability.
Ti SC, Alushin GM, Kapoor TM
Dev Cell 47 175-190.e5 (2018)
PMID: 30245156
Related structures: EMD-8997

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159369 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin beta-3 chain Chain: B
Molecule details ›
Chain: B
Length: 426 amino acids
Theoretical weight: 47.81 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q13509 (Residues: 1-426; Coverage: 95%)
Gene names: TUBB3, TUBB4
Sequence domains:
Structure domains:
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 48.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P68363 (Residues: 1-437; Coverage: 97%)
Gene name: TUBA1B
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand G2P 1 x G2P
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-8997
Expression system: Trichoplusia ni

Quick links

Citations

14 review citations

The tubulin code and its role in controlling microtubule properties and functions.
Janke et al. (2020)
13 more

5 mentions without citation

Reconstituting Microtubules: A Decades-Long Effort From Building Block Identification to the Generation of Recombinant α/β-Tubulin.
Ti SC. (2022)
4 more