› 6edb

X-ray diffraction
3.21Å resolution

Crystal structure of SRY.hcGAS-21bp dsDNA complex

Released:
DOI: 10.2210/pdb6edb/pdb
PDB entry 6edb coloured by chain, front view.
PDB entry 6edb coloured by chain, side view.
PDB entry 6edb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human cGAS catalytic domain has an additional DNA-binding interface that enhances enzymatic activity and liquid-phase condensation.
Xie W, Lama L, Adura C, Tomita D, Glickman JF, Tuschl T, Patel DJ
Proc Natl Acad Sci U S A 116 11946-11955 (2019)
PMID: 31142647

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119838 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cyclic GMP-AMP synthase; Sex-determining region Y protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 53.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05066 (Residues: 56-130; Coverage: 37%)
  • Canonical: Q8N884 (Residues: 157-522; Coverage: 70%)
Gene names: C6orf150, CGAS, MB21D1, SRY, TDF
Sequence domains:
Structure domains:
DNA (5'-D(*CP*GP*AP*TP*CP*CP*GP*GP*GP*AP*TP*CP*TP*AP*AP*AP*CP*AP*AP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
DNA (5'-D(*GP*CP*AP*TP*TP*GP*TP*TP*TP*AP*GP*AP*TP*CP*CP*CP*GP*GP*AP*TP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.76 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 90.307Å b: 115.827Å c: 159.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.255 0.302
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

45 review citations

Molecular mechanisms and cellular functions of cGAS-STING signalling.
Hopfner et al. (2020)
44 more

4 mentions without citation

Nuclear cGAS: guard or prisoner?
de Oliveira Mann et al. (2021)
3 more