› 6efk

X-ray diffraction
1.5Å resolution

Crystal structure of the human CHIP TPR domain in complex with a 5mer acetylated HSP70 peptide

Released:
DOI: 10.2210/pdb6efk/pdb
PDB entry 6efk coloured by chain, front view.
PDB entry 6efk coloured by chain, side view.
PDB entry 6efk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Specificity for latent C termini links the E3 ubiquitin ligase CHIP to caspases.
Ravalin M, Theofilas P, Basu K, Opoku-Nsiah KA, Assimon VA, Medina-Cleghorn D, Chen YF, Bohn MF, Arkin M, Grinberg LT, Craik CS, Gestwicki JE
Nat Chem Biol 15 786-794 (2019)
PMID: 31320752

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166669 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase CHIP Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UNE7 (Residues: 23-154; Coverage: 44%)
Gene names: CHIP, PP1131, STUB1
Sequence domains: Anaphase-promoting complex, cyclosome, subunit 3
ACE-ILE-GLU-GLU-VAL-ASP Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 630 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 46.709Å b: 70.342Å c: 77.072Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

HSP70 Multi-Functionality in Cancer.
Albakova et al. (2020)
9 more

4 mentions without citation

Exploration of Aberrant E3 Ligases Implicated in Alzheimer's Disease and Development of Chemical Tools to Modulate Their Function.
Potjewyd et al. (2021)
3 more