› 6ehb

X-ray diffraction
1.55Å resolution

OmpU, an outer membrane protein, of Vibrio cholerae

Released:
DOI: 10.2210/pdb6ehb/pdb
PDB entry 6ehb coloured by chain, front view.
PDB entry 6ehb coloured by chain, side view.
PDB entry 6ehb coloured by chain, top view.
Source organism: Vibrio cholerae O395
Primary publication:
Unusual Constriction Zones in the Major Porins OmpU and OmpT from Vibrio cholerae.
Pathania M, Acosta-Gutierrez S, Bhamidimarri SP, Baslé A, Winterhalter M, Ceccarelli M, van den Berg B
Structure 26 708-721.e4 (2018)
PMID: 29657131

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein U Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 320 amino acids
Theoretical weight: 34.69 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5F934 (Residues: 22-341; Coverage: 100%)
Gene names: VC0395_A0162, VC395_0650, ompU
Sequence domains: Gram-negative porin

Ligands and Environments

1 bound ligand:
Ligand C8E 23 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 62.723Å b: 153.84Å c: 66.17Å
α: 90° β: 102.32° γ: 90°
R-values:
R R work R free
0.172 0.17 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Gating of β-Barrel Protein Pores, Porins, and Channels: An Old Problem with New Facets.
Mayse et al. (2023)
7 other articles

4 mentions without citation

Water in Nanopores and Biological Channels: A Molecular Simulation Perspective.
Lynch et al. (2020)
3 more