› 6ehc

X-ray diffraction
2.02Å resolution

OmpUdeltaN (N-terminus deletion mutant of OmpU), outer membrane protein of Vibrio cholerae

Released:
DOI: 10.2210/pdb6ehc/pdb
Model geometry
Fit model/data
PDB entry 6ehc coloured by chain, front view.
PDB entry 6ehc coloured by chain, side view.
PDB entry 6ehc coloured by chain, top view.
Source organism: Vibrio cholerae O395
Primary publication:
Unusual Constriction Zones in the Major Porins OmpU and OmpT from Vibrio cholerae.
Pathania M, Acosta-Gutierrez S, Bhamidimarri SP, Baslé A, Winterhalter M, Ceccarelli M, van den Berg B
Structure 26 708-721.e4 (2018)
PMID: 29657131

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein U Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 33.64 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5F934 (Residues: 33-341; Coverage: 97%)
Gene names: VC0395_A0162, VC395_0650, ompU
Sequence domains: Gram-negative porin

Ligands and Environments

1 bound ligand:
Ligand C8E 10 x C8E
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P63
Unit cell:
a: 81.36Å b: 81.36Å c: 198.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.248
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Gating of β-Barrel Protein Pores, Porins, and Channels: An Old Problem with New Facets.
Mayse et al. (2023)
10 other articles