› 6ei0

X-ray diffraction
1.34Å resolution

Cytosolic copper storage protein Csp from Streptomyces lividans: apo form

Released:
DOI: 10.2210/pdb6ei0/pdb
PDB entry 6ei0 coloured by chain, front view.
PDB entry 6ei0 coloured by chain, side view.
PDB entry 6ei0 coloured by chain, top view.
Source organism: Streptomyces lividans 1326
Primary publication:
A cytosolic copper storage protein provides a second level of copper tolerance in Streptomyces lividans.
Straw ML, Chaplin AK, Hough MA, Paps J, Bavro VN, Wilson MT, Vijgenboom E, Worrall JAR
Metallomics 10 180-193 (2018)
PMID: 29292456

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Four-helix bundle copper-binding protein Chains: G, N, Q, U
Molecule details ›
Chains: G, N, Q, U
Length: 119 amino acids
Theoretical weight: 12.48 KDa
Source organism: Streptomyces lividans 1326
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1H2BDT0 (Residues: 17-135; Coverage: 88%)
Gene name: SAMN05428941_3460
Sequence domains: Domain of Unknown Function (DUF326)

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P6122
Unit cell:
a: 93.576Å b: 93.576Å c: 213.252Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Cu Homeostasis in Bacteria: The Ins and Outs.
Andrei et al. (2020)
4 more

1 mention without citation

Malaria parasite translocon structure and mechanism of effector export.
Ho et al. (2018)