› 6eig

X-ray diffraction
2.7Å resolution

Crystal structure of N24Q/C128T mutant of Channelrhodopsin 2

Released:
DOI: 10.2210/pdb6eig/pdb
PDB entry 6eig coloured by chain, front view.
PDB entry 6eig coloured by chain, side view.
PDB entry 6eig coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structural insights into ion conduction by channelrhodopsin 2.
Volkov O, Kovalev K, Polovinkin V, Borshchevskiy V, Bamann C, Astashkin R, Marin E, Popov A, Balandin T, Willbold D, Büldt G, Bamberg E, Gordeliy V
Science 358 (2017)
PMID: 29170206

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensory opsin B Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 35.21 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Leishmania tarentolae
UniProt:
  • Canonical: Q8RUT8 (Residues: 1-315; Coverage: 43%)
Gene names: CHLRE_02g085257v5, CSOB, acop2, cop4
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

3 bound ligands:
Ligand OLC 7 x OLC
Ligand PO4 2 x PO4
Ligand LFA 2 x LFA
1 modified residue:
Ligand LYR 2 x LYR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 64.51Å b: 134.06Å c: 78.47Å
α: 90° β: 90.22° γ: 90°
R-values:
R R work R free
0.266 0.264 0.298
Expression system: Leishmania tarentolae

Quick links

Citations

10 review citations

Leishmania tarentolae: Taxonomic classification and its application as a promising biotechnological expression host.
Klatt et al. (2019)
9 more

1 mention without citation

a-ARM: Automatic Rhodopsin Modeling with Chromophore Cavity Generation, Ionization State Selection, and External Counterion Placement.
Pedraza-González et al. (2019)