› 6eji

X-ray diffraction
2.3Å resolution

Structure of a glycosyltransferase

Released:
DOI: 10.2210/pdb6eji/pdb
PDB entry 6eji coloured by chain, front view.
PDB entry 6eji coloured by chain, side view.
PDB entry 6eji coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase.
Ramírez AS, Boilevin J, Mehdipour AR, Hummer G, Darbre T, Reymond JL, Locher KP
Nat Commun 9 445 (2018)
PMID: 29386647

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WlaC protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 43.26 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: O86151 (Residues: 1-358; Coverage: 100%)
Gene name: wlaC
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand UD2 2 x UD2
Ligand K 2 x K
Ligand GOL 2 x GOL
Ligand NA 4 x NA
Ligand CL 3 x CL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 61.62Å b: 127.03Å c: 71.58Å
α: 90° β: 90.13° γ: 90°
R-values:
R R work R free
0.197 0.196 0.223
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The surprising structural and mechanistic dichotomy of membrane-associated phosphoglycosyl transferases.
O'Toole et al. (2021)
10 other articles

5 mentions without citation

Monotopic Membrane Proteins Join the Fold.
Allen et al. (2019)
4 more