› 6ek2

X-ray diffraction
2.65Å resolution

CRYSTAL STRUCTURE OF HUMAN CD81 LARGE EXTRACELLULAR LOOP IN COMPLEX WITH SINGLE CHAIN FV FRAGMENT 10

Released:
DOI: 10.2210/pdb6ek2/pdb
PDB entry 6ek2 coloured by chain, front view.
PDB entry 6ek2 coloured by chain, side view.
PDB entry 6ek2 coloured by chain, top view.
Source organisms:
Primary publication:
Structure-Guided Combinatorial Engineering Facilitates Affinity and Specificity Optimization of Anti-CD81 Antibodies.
Nelson B, Adams J, Kuglstatter A, Li Z, Harris SF, Liu Y, Bohini S, Ma H, Klumpp K, Gao J, Sidhu SS
J Mol Biol 430 2139-2152 (2018)
PMID: 29778602

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211409 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CD81 antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 10.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60033 (Residues: 112-201; Coverage: 38%)
Gene names: CD81, TAPA1, TSPAN28
Sequence domains: Tetraspanin family
Structure domains: Tetraspanin
SINGLE CHAIN FV FRAGMENT Chains: H, I
Molecule details ›
Chains: H, I
Length: 247 amino acids
Theoretical weight: 26.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 52.317Å b: 118.06Å c: 130.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.247
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Antibody Structure and Function: The Basis for Engineering Therapeutics.
Chiu et al. (2019)
2 more

1 mention without citation

A dynamic interaction between CD19 and the tetraspanin CD81 controls B cell co-receptor trafficking.
Susa et al. (2020)