› 6ek9

X-ray diffraction
1.5Å resolution

Cytosolic copper storage protein Csp from Streptomyces lividans: Cu loaded form

Released:
DOI: 10.2210/pdb6ek9/pdb
PDB entry 6ek9 coloured by chain, front view.
PDB entry 6ek9 coloured by chain, side view.
PDB entry 6ek9 coloured by chain, top view.
Source organism: Streptomyces lividans
Primary publication:
A cytosolic copper storage protein provides a second level of copper tolerance in Streptomyces lividans.
Straw ML, Chaplin AK, Hough MA, Paps J, Bavro VN, Wilson MT, Vijgenboom E, Worrall JAR
Metallomics 10 180-193 (2018)
PMID: 29292456

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 12.62 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6ES11 (Residues: 16-136; Coverage: 89%)
Gene name: SSPG_04277
Sequence domains: Domain of Unknown Function (DUF326)

Ligands and Environments

1 bound ligand:
Ligand CU1 20 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 62.081Å b: 64.138Å c: 66.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Cu Homeostasis in Bacteria: The Ins and Outs.
Andrei et al. (2020)
4 more