› 6emk

Electron Microscopy
8Å resolution

Cryo-EM Structure of Saccharomyces cerevisiae Target of Rapamycin Complex 2

Released:
DOI: 10.2210/pdb6emk/pdb
PDB entry 6emk coloured by chain, front view.
PDB entry 6emk coloured by chain, side view.
PDB entry 6emk coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structure of Saccharomyces cerevisiae target of rapamycin complex 2.
Karuppasamy M, Kusmider B, Oliveira TM, Gaubitz C, Prouteau M, Loewith R, Schaffitzel C
Nat Commun 8 1729 (2017)
PMID: 29170376
Related structures: EMD-3896

Function and Biology Details

Reactions catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-152391 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Serine/threonine-protein kinase TOR2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 2474 amino acids
Theoretical weight: 281.92 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32600 (Residues: 1-2474; Coverage: 100%)
Gene names: DRR2, TOR2, TSC14, YKL203C
Sequence domains:
Target of rapamycin complex subunit LST8 Chains: B, D
Molecule details ›
Chains: B, D
Length: 303 amino acids
Theoretical weight: 34.08 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P41318 (Residues: 1-303; Coverage: 100%)
Gene names: LST8, N2005, YNL006W
Sequence domains: WD domain, G-beta repeat
Target of rapamycin complex 2 subunit TSC11 Chains: E, F
Molecule details ›
Chains: E, F
Length: 303 amino acids
Theoretical weight: 25.8 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
Target of rapamycin complex 2 subunit AVO2 Chains: G, H
Molecule details ›
Chains: G, H
Length: 426 amino acids
Theoretical weight: 47.21 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04749 (Residues: 1-426; Coverage: 100%)
Gene names: AVO2, YM9916.07, YMR068W
Sequence domains: Ankyrin repeat
Target of rapamycin complex 2 subunit AVO1 Chains: I, J
Molecule details ›
Chains: I, J
Length: 1176 amino acids
Theoretical weight: 131.57 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q08236 (Residues: 1-1176; Coverage: 100%)
Gene names: AVO1, O1110, YOL078W
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8Å
Relevant EMDB volumes: EMD-3896
Expression system: Saccharomyces cerevisiae

Quick links

Citations

10 review citations

Regulation and metabolic functions of mTORC1 and mTORC2.
Szwed et al. (2021)
9 more

3 mentions without citation

Structural Insights into TOR Signaling.
Tafur et al. (2020)
2 more