› 6eoj

Electron Microscopy
3.55Å resolution

PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb6eoj/pdb
PDB entry 6eoj coloured by chain, front view.
PDB entry 6eoj coloured by chain, side view.
PDB entry 6eoj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Architecture of eukaryotic mRNA 3'-end processing machinery.
Casañal A, Kumar A, Hill CH, Easter AD, Emsley P, Degliesposti G, Gordiyenko Y, Santhanam B, Wolf J, Wiederhold K, Dornan GL, Skehel M, Robinson CV, Passmore LA
Science 358 1056-1059 (2017)
PMID: 29074584
Related structures: EMD-3908

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-154893 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein CFT1 Chain: A
Molecule details ›
Chain: A
Length: 1357 amino acids
Theoretical weight: 153.58 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q06632 (Residues: 1-1357; Coverage: 100%)
Gene names: CFT1, YDR301W, YHH1
Sequence domains: CPSF A subunit region
mRNA 3'-end-processing protein YTH1 Chain: B
Molecule details ›
Chain: B
Length: 208 amino acids
Theoretical weight: 24.56 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q06102 (Residues: 2-208; Coverage: 100%)
Gene names: YPR107C, YTH1
Sequence domains:
Polyadenylation factor subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 470 amino acids
Theoretical weight: 53.64 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42841 (Residues: 1-465; Coverage: 100%)
Gene names: N0348, PFS2, YNL317W
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.55Å
Relevant EMDB volumes: EMD-3908
Expression system: Spodoptera frugiperda

Quick links

Citations

18 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
17 more

7 mentions without citation

Mechanistic insights into mRNA 3'-end processing.
Kumar et al. (2019)
6 more