› 6ep6

X-ray diffraction
1.59Å resolution

ARABIDOPSIS THALIANA GSTU23, reduced

Released:
DOI: 10.2210/pdb6ep6/pdb
PDB entry 6ep6 coloured by chain, front view.
PDB entry 6ep6 coloured by chain, side view.
PDB entry 6ep6 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Disulfide bond formation protects Arabidopsis thaliana glutathione transferase tau 23 from oxidative damage.
Tossounian MA, Van Molle I, Wahni K, Jacques S, Gevaert K, Van Breusegem F, Vertommen D, Young D, Rosado LA, Messens J
Biochim Biophys Acta Gen Subj 1862 775-789 (2018)
PMID: 29031766

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase U23 Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 25.71 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9M9F1 (Residues: 1-220; Coverage: 100%)
Gene names: At1g78320, F3F9.14, GSTU23
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 88.597Å b: 50.449Å c: 113.702Å
α: 90° β: 108.66° γ: 90°
R-values:
R R work R free
0.19 0.189 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Reactive oxygen species signalling in plant stress responses.
Mittler et al. (2022)
1 more

1 mention without citation

Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases.
Sylvestre-Gonon et al. (2019)