› 6eu9

X-ray diffraction
2.69Å resolution

Crystal structure of Platynereis dumerilii RAR ligand-binding domain in complex with all-trans retinoic acid

Released:
DOI: 10.2210/pdb6eu9/pdb
PDB entry 6eu9 coloured by chain, front view.
PDB entry 6eu9 coloured by chain, side view.
PDB entry 6eu9 coloured by chain, top view.
Source organism: Platynereis dumerilii
Primary publication:
The ancestral retinoic acid receptor was a low-affinity sensor triggering neuronal differentiation.
Handberg-Thorsager M, Gutierrez-Mazariegos J, Arold ST, Kumar Nadendla E, Bertucci PY, Germain P, Tomançak P, Pierzchalski K, Jones JW, Albalat R, Kane MA, Bourguet W, Laudet V, Arendt D, Schubert M
Sci Adv 4 eaao1261 (2018)
PMID: 29492455

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104735 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NR LBD domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 241 amino acids
Theoretical weight: 27.46 KDa
Source organism: Platynereis dumerilii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A384E144 (Residues: 1-241; Coverage: 100%)
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand REA 2 x REA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 187.41Å b: 68.15Å c: 95.08Å
α: 90° β: 100.75° γ: 90°
R-values:
R R work R free
0.257 0.255 0.3
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Retinoic acid signaling pathways.
Ghyselinck et al. (2019)
4 more