› 6euw

X-ray diffraction
1Å resolution

Crystal structure of the cap-binding domain of the PB2 subunit of influenza A/H5N1 polymerase bound to an azaindazole inhibitor

Released:
DOI: 10.2210/pdb6euw/pdb
PDB entry 6euw coloured by chain, front view.
PDB entry 6euw coloured by chain, side view.
PDB entry 6euw coloured by chain, top view.
Source organism: Influenza A virus (A/duck/Shantou/4610/2003(H5N1))
Primary publication:
Capped RNA primer binding to influenza polymerase and implications for the mechanism of cap-binding inhibitors.
Pflug A, Gaudon S, Resa-Infante P, Lethier M, Reich S, Schulze WM, Cusack S
Nucleic Acids Res 46 956-971 (2018)
PMID: 29202182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173705 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.25 KDa
Source organism: Influenza A virus (A/duck/Shantou/4610/2003(H5N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2LG68 (Residues: 318-483; Coverage: 21%)
Gene name: PB2
Sequence domains: Influenza RNA polymerase PB2 CAP binding domain

Ligands and Environments

2 bound ligands:
Ligand BYB 1 x BYB
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P61
Unit cell:
a: 62.49Å b: 62.49Å c: 69.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.141 0.139 0.164
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure and Function of the Influenza Virus Transcription and Replication Machinery.
Fodor et al. (2020)
6 more

2 mentions without citation

Capped RNA primer binding to influenza polymerase and implications for the mechanism of cap-binding inhibitors.
Pflug et al. (2018)
1 more