› 6eux

X-ray diffraction
2.05Å resolution

Structure of the midlink and cap-binding domains of influenza B polymerase PB2 subunit with a bound azaindazole cap-binding inhibitor

Released:
DOI: 10.2210/pdb6eux/pdb
PDB entry 6eux coloured by chain, front view.
PDB entry 6eux coloured by chain, side view.
PDB entry 6eux coloured by chain, top view.
Source organism: Influenza B virus (B/Memphis/13/2003)
Primary publication:
Capped RNA primer binding to influenza polymerase and implications for the mechanism of cap-binding inhibitors.
Pflug A, Gaudon S, Resa-Infante P, Lethier M, Reich S, Schulze WM, Cusack S
Nucleic Acids Res 46 956-971 (2018)
PMID: 29202182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase basic protein 2 Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.01 KDa
Source organism: Influenza B virus (B/Memphis/13/2003)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5V8X3 (Residues: 249-540; Coverage: 38%)
Gene name: PB2
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand BYB 1 x BYB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P3121
Unit cell:
a: 73.2Å b: 73.2Å c: 103.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.229 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Structure and Function of the Influenza Virus Transcription and Replication Machinery.
Fodor et al. (2020)
5 more

3 mentions without citation

Revisiting Viral RNA-Dependent RNA Polymerases: Insights from Recent Structural Studies.
Ramaswamy et al. (2022)
2 more