› 6ewn

X-ray diffraction
2.29Å resolution

HspA from Thermosynechococcus vulcanus in the presence of 2M urea with initial stages of denaturation

Released:
DOI: 10.2210/pdb6ewn/pdb
PDB entry 6ewn coloured by chain, front view.
PDB entry 6ewn coloured by chain, side view.
PDB entry 6ewn coloured by chain, top view.
Source organism: Thermostichus vulcanus
Primary publication:
Biophysical and structural characterization of the small heat shock protein HspA from Thermosynechococcus vulcanus in 2 M urea.
Ghosh S, Salama F, Dines M, Lahav A, Adir N
Biochim Biophys Acta Proteins Proteom 1867 442-452 (2019)
PMID: 30711645

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SHSP domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 11.13 KDa
Source organism: Thermostichus vulcanus
Expression system: Escherichia coli
UniProt:
  • Canonical: O82825 (Residues: 37-135; Coverage: 68%)
Gene name: hspA
Sequence domains: Hsp20/alpha crystallin family
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand URE 44 x URE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I41
Unit cell:
a: 88.65Å b: 88.65Å c: 114.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.28 0.28 0.342
Expression system: Escherichia coli

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