› 6ewx

X-ray diffraction
2.77Å resolution

Structure of Pragmin pseudo-kinase reveals a dimerization mechanism to regulate protein tyrosine phosphorylation and nuclear transcription

Released:
DOI: 10.2210/pdb6ewx/pdb
PDB entry 6ewx coloured by chain, front view.
PDB entry 6ewx coloured by chain, side view.
PDB entry 6ewx coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Dimerization of the Pragmin Pseudo-Kinase Regulates Protein Tyrosine Phosphorylation.
Lecointre C, Simon V, Kerneur C, Allemand F, Fournet A, Montarras I, Pons JL, Gelin M, Brignatz C, Urbach S, Labesse G, Roche S
Structure 26 545-554.e4 (2018)
PMID: 29503074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inactive tyrosine-protein kinase PRAG1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 484 amino acids
Theoretical weight: 53.86 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: D3ZMK9 (Residues: 906-1368; Coverage: 34%)
Gene names: Prag1, Pragmin
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 193.43Å b: 58.92Å c: 110.64Å
α: 90° β: 118.48° γ: 90°
R-values:
R R work R free
0.19 0.188 0.246
Expression system: Escherichia coli K-12

Quick links

Citations

4 review citations

There's more to death than life: Noncatalytic functions in kinase and pseudokinase signaling.
Mace et al. (2021)
3 more

4 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
3 more