› 6ex3

X-ray diffraction
2.2Å resolution

Staphylococcus aureus superoxide dismutase SodA

Released:
DOI: 10.2210/pdb6ex3/pdb
PDB entry 6ex3 coloured by chain, front view.
PDB entry 6ex3 coloured by chain, side view.
PDB entry 6ex3 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
An evolutionary path to altered cofactor specificity in a metalloenzyme
Barwinska-Sendra A, Garcia YM, Sendra K, Basle A, Mackenzie ES, Tarrant E, Card P, Tabares LC, Bicep C, Un S, Kehl-Fie TE, Waldron KJ
Nat Commun (2020)

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn] 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 199 amino acids
Theoretical weight: 22.74 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0J3 (Residues: 1-199; Coverage: 100%)
Gene names: SAOUHSC_01653, sodA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE 4 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 47.557Å b: 66.381Å c: 108.311Å
α: 90° β: 98.72° γ: 90°
R-values:
R R work R free
0.204 0.201 0.267
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere.
Van Stappen et al. (2022)
4 more

1 mention without citation

An evolutionary path to altered cofactor specificity in a metalloenzyme.
Barwinska-Sendra et al. (2020)