› 6exl

X-ray diffraction
1.9Å resolution

The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with a ring-fused 2-pyridone (MK206) - folded HTH motif

Released:
DOI: 10.2210/pdb6exl/pdb
PDB entry 6exl coloured by chain, front view.
PDB entry 6exl coloured by chain, side view.
PDB entry 6exl coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
Structure-Based Design of Inhibitors Targeting PrfA, the Master Virulence Regulator of Listeria monocytogenes.
Kulén M, Lindgren M, Hansen S, Cairns AG, Grundström C, Begum A, van der Lingen I, Brännström K, Hall M, Sauer UH, Johansson J, Sauer-Eriksson AE, Almqvist F
J Med Chem 61 4165-4175 (2018)
PMID: 29667825

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.53 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 1-237; Coverage: 100%)
Gene names: lmo0200, prfA
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand C2Q 2 x C2Q
Ligand NA 3 x NA
Ligand IPA 2 x IPA
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 47.749Å b: 87.795Å c: 116.183Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.225
Expression system: Escherichia coli

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Citations

1 review citation

Long Chain Fatty Acids and Virulence Repression in Intestinal Bacterial Pathogens.
Mitchell et al. (2022)
10 other articles