Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- RNA polymerase sigma-70
- DNA-directed RNA polymerase, insert domain superfamily
- DNA-directed RNA polymerase, RpoA/D/Rpb3-type
- RNA polymerase, RBP11-like subunit
- DNA-directed RNA polymerase, alpha subunit
- DNA-directed RNA polymerase, omega subunit
- Winged helix-like DNA-binding domain superfamily
- RNA polymerase sigma factor, region 3/4-like
36 more domains
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-106290 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
DNA-directed RNA polymerase subunit alpha
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 37.96 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 350 amino acids
Theoretical weight: 37.96 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
- Canonical:
A0QSL8 (Residues: 1-350; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta
Molecule details ›
Chain: C
Length: 1178 amino acids
Theoretical weight: 129.46 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 1178 amino acids
Theoretical weight: 129.46 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
- Canonical: P60281 (Residues: 2-1169; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta'
Molecule details ›
Chain: D
Length: 1325 amino acids
Theoretical weight: 147.79 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 1325 amino acids
Theoretical weight: 147.79 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
- Canonical: A0QS66 (Residues: 2-1317; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit omega
Molecule details ›
Chain: E
Length: 107 amino acids
Theoretical weight: 11.51 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
Sequence domains: RNA polymerase Rpb6
Length: 107 amino acids
Theoretical weight: 11.51 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
- Canonical: A0QWT1 (Residues: 2-107; Coverage: 99%)
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA
Molecule details ›
Chain: F
Length: 466 amino acids
Theoretical weight: 51.57 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 466 amino acids
Theoretical weight: 51.57 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
- Canonical: A0QW02 (Residues: 1-466; Coverage: 100%)
Sequence domains:
